Suthindhiran K

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Jacob J.J

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Biotechnology Reports

In this work, the effect of particle size on alkali pretreatment of the almond shell was evaluated for recovery of hemicellulose. Further, endoxylanase from Thermomyces lanuginosus was immobilized on Fe-based magnetic nanoparticles to enable reuse of enzyme. Reduction in particle size significantly influences the recovery of hemicellulose as particle size below 120 μm enable recovery of 97% available hemicellulose in 1 h at 121 °C with 2 M alkali. The enzyme could retain 93.3% of enzymatic activity upon immobilization onto magnetic support using glutaraldehyde (25 mM) and was at par with the free enzyme in terms of pH and temperature profile. The measurement of reaction kinetics (Km and Vmax) indicates similar values for free and immobilized enzyme. The structural and morphological analysis indicates presence near spherical magnetic core and successful cross-linking of the enzyme without alteration of the magnetic core. The immobilized enzyme was able to hydrolyze hemicellulose to produce XOS, the yield equivalent to 67.4% of that obtained using free enzyme at 50 °C. The comparison of XOS production ability at 50 and 60 °C, suggests that the immobilized enzyme retains activity as similar yield was obtained at both temperatures, whereas, the yield for free enzyme decreases significantly. The XOS yield on recycling of immobilized enzyme for three successive cycles was found to reduce to 41% of the initial cycle. However, in all cycles of enzymatic hydrolysis, the percentage of xylobiose was found to be above 90%. © 2019

Enzyme and Microbial Technology

Low degree of polymerization xylooligosaccharides production from almond shell using immobilized nano-biocatalyst

International Journal of Biological Macromolecules

Magnetic nanoparticles as versatile carriers for enzymes immobilization: A review

Journal of Applied Polymer Science

Pilot-scale development of core-shell polymer supports for the immobilization of recombinant lipase B fromCandida antarcticaand their application in the production of ethyl esters from residual fatty acids

Catalysts

A General Overview of Support Materials for Enzyme Immobilization: Characteristics, Properties, Practical Utility

Biotechnology and Applied Biochemistry

A review on the important aspects of lipase immobilization on nanomaterials

Immobilisation of lipase enzyme onto bacterial magnetosomes for stain removal

Journal	Data powered by TypesetBiotechnology Reports
Publisher	Data powered by TypesetElsevier BV
ISSN	2215-017X
Open Access	Yes