Interface tissues are specialized and ubiquitous tissues in human body, which integrate soft and hard tissues, and are characterized by their complex and heterogeneous biochemical and structural properties. Fibrocartilage is a notable example for an interface tissue, which integrates anterior cruciate ligament with bone tissue. Interface tissues are challenging to repair after injuries and current clinical methods of fixation and tissue regeneration have not resulted in full recovery of damaged tissues. Recent studies of interface tissue engineering (ITE) have exploited the use of gradient biomaterials as biomimetic scaffolds to help regenerating biological functions of native tissues. In this article, the authors review the recent developments and impact of gradient biomaterials on ITE. In addition, a brief introduction of gradients and interface tissues, and the main challenges of current clinical methods of regenerating interface tissues are also discussed. Finally, the role of gradient biomaterials as emerging platforms to address these issues is reviewed. © 2012 American Scientific Publishers. All rights reserved.