Homo sapiens and Mus musculus genomes have the similarity ranges from 70% to 90% with an average of 85%. Interleukin (IL) has 17 different families among which interleukin 2 (IL2) is present in both Homo sapiens and Mus musculus. IL2 activates the receptor named IL2-R which further proliferate lymphocytes such as T-cell and B-cell. IL2 is a glycosylated polypeptide lymphokine. It requires cleavage of the signal sequence for its activity. IL2 is used for immunotherapy to treat cancer and its suppression avoids autoimmunity in patients with transplantations. IL2 of Homo sapiens and Mus musculus are studied and analysed using simulation and structural analysis methods. The results are compared to obtain their similarity profile. This result can further be applied in the study of inflammatory disease related aspects in future.