The antibacterial activities of four honey samples collected from different locations of Vellore district and one sample from New Zealand named manuka honey were all tested against clinical pathogens such as Staphylococcus aureus and Klebsiella pneumoniae. These honey samples were compared with standard antibiotics like Ampicillin, Tetracycline, Chloramphenicol and Erythromycin. The antibacterial activity was tested using Kirby-Bauer's method for antibiotics and well diffusion method for honey samples. The honey samples were tested at concentrations of 25, 40, 50, 75% and net honey i.e. 100%. Undiluted honey samples inhibited the growth of all the strains. All diluted honey samples inhibited the growth of Staphylococcus aureus MTCC 737, Staphylococcus aureus from sputum and BAL at varying concentrations whereas, honey at higher concentrations was required to inhibit the growth of Klebsiella pneumoniae. None of the strains were inhibited at 25% concentration.