Over the last few years researchers from all over the world have aimed at identifying and isolating plant derived substances for the treatment of various diseases. The synthetic products of the modern medicine surpassed the importance of medicinal plants for a while and moreover they are toxic and possess various side effects. Now people turning back to the natural drugs of plant origin with the hope of safety and efficacy. In the present work betacyanins were isolated from the bracts of Bougainvillea glabra by using flash chromatography and the isolated betacyanins were characterized by FT-IR, Mass Specrtoscopy. Betacyacins were screened for their in-vitro antioxidant activity by DPPH radical scavenging assay using ascorbic as standard and antibacterial studies were also carried out by well-diffusion method using ampicillin as standard. Results obtained showed that betacyanins posess good radical scavenging activity with 75.92 percent inhibition at 100 μg/ml of sample concentration. In-vitro antibacterial studies showed that betacyanins possess better antibacterial efficacy against Bacillus subtilis and Pseudomonas aeroginosa.