Scoparia dulcis is a perennial multi-purpose medicinal herb distributed throughout tropical and subtropical regions which belongs to the family Scrophulariaceae. The purpose of the present study is to explore the Scoparia dulcis germplasm by means of in vitro polyploidization in order to improve their regeneration capabilities. The Murashige- Skoog medium, supplemented with 0.5 mg/l KIN and 2.0 mg/l IAA was used for simultaneous regeneration of the nodal segments of S. dulcis. The colchicine doses tested with S. dulcis were: 0.0; 0.0001; 0.005; 0.001 and 0.01 for 48 hrs. Significant differences were observed for the morphology of flower, leaves, and the stem among the colchicinetreated plants and the control. Best results were observed with 0.001 % colchicine. The tissue culture proved to be a powerful tool both to multiply the Scoparia material incorporated to our germplasm collection and to obtain new improved varieties of this medicinal herb. © RJPT All right reserved.