Bromelain refers to a family of proteolytic enzymes containing sulfhydryl group obtained from the Pineapple plant (Ananas comosus). The objective of this study was to determine the optimum pH and temperature for the hemolytic activity of the extracts from various parts of the Pineapple fruit and to test the clot buster activity. The crude extracts were partially purified by ammonium sulphate precipitation followed by dialysis and protease assay was performed. The partially purified extracts were tested for hemolytic activity for a range of temperature (4°C-95°C) and pH (pH 4-9). The optimum activity was observed at 9 pH and 45°C. The extracts with maximum hemolytic activity were assessed for clot buster activity. Extract SP-50 from stem pellet showed the highest clot buster activity of 68%. The results indicate a potential use of the pineapple extracts for clot dissolution in the pharmaceutical, tannery and food industry in the near future. © 2016, Global Research Online. All rights reserved.