In the present study, an attempt has been made to evaluate the immunomodulatory effects of the Indian ayurvedic herbal formulation Triphala on experimental induced inflammation. The effect of Triphala was investigated on complement activity, humoral immune response, and cell mediated immune response in mice, and in mitogen (phytoheamagglutinin)-induced T-lymphocyte proliferation in vitro. Triphala administration significantly inhibited the complement activity, humoral and cell mediated immune response (delayed type hypersensitivity reaction (DTH)), and mitogen (phytohaemagglutinin)-induced T-lymphocyte proliferation in a dose dependent manner. These observations suggest that Triphala caused immunosuppression in experimental-induced inflammation, indicating that they may provide an alternative approach to the treatment of inflammatory and autoimmune diseases.