In this chapter, we investigate the impact of induced pluripotent stem cells (iPSCs) in scaffold-based tissue engineering and its potential for tissue regenerative medicine. Scaffold-based tissue engineering aims to develop functionalized tissues/organs for repair and regeneration of damaged or diseased body parts by employing engineered matrices called scaffolds cultured with cells and bioactive molecules. iPSCs are adult cells that have been genetically reprogrammed into an embryonic-cell-like state. Owing to their unique regenerative capacity, iPSCs have attracted the attention of the biomedical community and demonstrated their capability as an invaluable cell source for cell therapy and for scaffold-based tissue engineering. However, the underlying mechanism of the interaction of iPSCs with three-dimensional (3D) scaffolds is unclear, which is very essential to realize the full potential of iPSCs in engineering functional tissues and organs. In this chapter, we therefore review the impact of iPSCs in scaffold-based tissue engineering and its potential for tissue regenerative medicine. The techniques employed for the culture of iPSCs into 3D scaffolds, the effect of the 3D microenvironment in controlling iPSCs' fate and function, and the potential applications of iPSCs in tissue engineering are discussed with illustrated examples. The chapter is expected to be useful for readers to gain insight into the impact of iPSCs in various 3D scaffold-based tissue engineering fields such as bone, cartilage, cardiac, skin, and neural tissue engineering. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA.