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Induction of Diploid Androgenesis in the Stinging Catfish,Heteropneustes fossilis
, Murugesan A.G, Sukumaran N.
Published in Wiley
Volume: 45
Issue: 5
Pages: 558 - 566
This study reports the results on induced diploid androgenesis in the Indian catfish, Heteropneustes fossilis. Ultraviolet (UV) irradiation was used to inactivate maternal genome of H. fossilis. Complete inactivation of maternal genome was recorded at 12,500ergs/mm2. These genome-inactivated eggs of H. fossilis were inseminated with conspecific sperm. The sperm suspension was diluted to 1×107spermml/L in Hank's Balanced Salt Solution. Egg viability was assessed for different exposure durations at fertilization, hatching, haploidy, and diploidization. Majority of the larvae derived from irradiated eggs had abnormal appearance. Complete inactivation of maternal genome was detected by haploid syndrome and confirmed by chromosome counting (n=29). These eggs activated with sperm were subjected to heat shock at 40 and 41C for different postactivation times and durations. Diploid androgens had a normal appearance as controls and confirmed by chromosome counting (n=58). A maximum of 21 and 14% of diploidization was recorded at 30min after activation, at 40 and 41C, which corresponds to the first cleavage suppression time. © the World Aquaculture Society 2014.
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JournalData powered by TypesetJournal of the World Aquaculture Society
PublisherData powered by TypesetWiley
Open Access0