The influence of tempering treatments on mechanical properties and hydrogen embrittlement (HE) of 13 wt% Cr martensitic stainless steel (MSS) have been investigated by Charpy impact tests and slow strain rate tensile (SSRT) tests followed by fracture surface examination. The austenitized and quenched specimens were tempered at 300, 550 and 700 °C for 2.5 h. The MSS tempered at 550 °C showed brittle intergranular (IG) fracture after impact tests indicating its susceptibility to temper embrittlement. The experimental results showed that the 13 wt% Cr MSS is sensitive to HE. The as-quenched condition showed cracking during hydrogen pre-charging itself. Hydrogen pre-charging duration increased the susceptibility to HE of tempered MSS. The maximum HE susceptibility was observed for specimen tempered at 550 °C with a drastic reduction in strength and strain to failure. Tempering at 300 and 550 °C showed brittle IG fracture with hydrogen pre-charging in SSRT tests whereas increased IG region with ductile dimples was observed for specimen tempered at 700 °C with increase of pre-charging duration. The reason for maximum susceptibility to HE of specimen tempered at 550 °C is due to synergistic interaction of hydrogen and impurities segregated at prior austenitic grain boundaries. © 2019 Elsevier Ltd