Soil sample was collected from the coastal region of Tamil Nadu with the aim of isolating actinobacteria and screen them for antagonistic activity against common bacterial and fungal pathogens. Serial dilution of the soil sample and subsequent screening of the isolates obtained, resulted in the identification of a potential strain VITDDK2 with significant activity against Klebsiella pneumoniae, Aspergillus flavus and Aspergillus niger. In addition, the strain VITDDK2 also possessed chitinolytic activity. Chemotaxonomic analysis showed that the isolate VITDDK2 belongs to cell wall Type I. 16 S rRNA partial gene sequence and phylogenetic analysis showed that the strain VITDDK2 shared 93% similarity with Streptomyces sp. strain 346. Also the secondary structure of the rRNA of VITDDK2 and the restriction sites were predicted using Genebee and NEBCutter softwares respectively. © 2010 Deepika L, et al.