Soil sample was collected from the coastal region of Tamil Nadu with the aim of isolating actinobacteria and screen them for antagonistic activity against common bacterial and fungal pathogens. Serial dilution of the soil sample and subsequent screening of the isolates obtained, resulted in the identification of a potential strain VITDDK2 with significant activity against Klebsiella pneumoniae, Aspergillus flavus and Aspergillus niger. In addition, the strain VITDDK2 also possessed chitinolytic activity. Chemotaxonomic analysis showed that the isolate VITDDK2 belongs to cell wall Type I. 16 S rRNA partial gene sequence and phylogenetic analysis showed that the strain VITDDK2 shared 93% similarity with Streptomyces sp. strain 346. Also the secondary structure of the rRNA of VITDDK2 and the restriction sites were predicted using Genebee and NEBCutter softwares respectively. © 2010 Deepika L, et al.

Kannabiran K

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Deepika L

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Isolation and Characterization of Antagonistic Actinomycetes from Marine Soil

Journal of Microbial & Biochemical Technology

Objective: This study was focused on an evaluation of antimicrobial activity of actinobacterial isolated from Kanyakumari marine sediments.Methods: This study includes sample collection from Kanyakumari beach, followed by isolation of marine actinobacteria, screening for antibacterial activity and antifungal activity. Finally, the most active isolate was identified using 16S rRNA sequencing. Results: Marine actinobacteria are one of the leading microbes of the ocean known for producing excellent secondary metabolites and having a broad spectrum of anti-microbial activity, hence they are very important from an industrial point of view. The marine sediment sample used was collected from Kanyakumari beach, in the month of July, falling in the latitudinal range of 8.5074°N and in the longitude of 76.9730°E. A total of 8 actinobacterial colonies were isolated by spread plate technique on Actinomycetes isolation agar, Kuster's agar and Starch casein agar. The isolate cultures were designated as UST1- UST8. Among them, UST3 showed a maximum zone of inhibition against clinical pathogens Salmonella typhi and Aspergillus niger with a zone of inhibition of 22mm and 20mm respectively. In solvent extraction study ethyl acetate and hexane was found as best solvents for extraction. Potential isolate UST3 was identified as Streptomyces variabilis using morphological studies and 16S rRNA sequencing. Potential strain was submitted as Streptomyces variabilis- VITUMVB03Conclusion: Results obtained in this study concluded that marine actinobacteria have broad-spectrum antimicrobial activity and can be used in the pharmaceutical industry.Keywords: Anti-fungal activity, Anti-bacterial activity, Salmonella typhi, Aspergillus niger, Streptomyces variabilis

Asian Journal of Pharmaceutical and Clinical Research

ANTIMICROBIAL ACTIVITY OF STREPTOMYCES VARIABILIS STRAIN-VITUMVB03 ISOLATED FROM KANYAKUMARI MARINE SEDIMENTS

Phosphomolybdic acid (PMo12) stabilized Nafion (Nf) membrane colloidal solution ({Nf-PMo12}) modified glassy carbon electrode (GCE/{Nf-PMo12}-CME) has been demonstrated as a new and efficient electrocatalyst for cysteine (CySH) oxidation in pH 7 phosphate buffer solution. Physico-chemical characterizations of the {Nf-PMo12} by TEM, XRD, FT-IR and UV-vis spectroscopy techniques revealed stabilization of the PMo12 as 5-60 nm sized nano-aggregates within the sulphonic acid micro-channels of Nafion without any changes in the crystal structure. Electrochemical characterization of the GCE/{Nf-PMo12}-CME with Fe(CN)63- and Ru(bpy)32+ indicates metal like surface feature of the working electrode. The GCE/{Nf-PMo12}-CME showed highly stable CySH electrochemical oxidation current signal at 0.58 ± 0.02 V vs. Ag/AgCl in pH 7 with 150 mV reduction in the response and about 8 times higher the peak current than that of the respective unmodified electrode. Amperometric i-t response of CySH at an applied potential of 0.58 V vs. Ag/AgCl yielded current sensitivity and regression coefficient values of 0.011 μA/μM and 0.9998 respectively without any interference from xanthine, hypoxanthine, nitrite, uric acid and other sulphur containing biochemicals; methionine and taurine, except ascorbic acid. Calculated detection limit value is (signal-to-noise ratio, S/N = 3) 25 μM. In particular, the PMo12 and {Nf-PMo12} systems showed marked anti-dermatophytic activity against Trichophyton rubrum organism, and the activity is comparable with standard fluconazole drug. Crown Copyright © 2013 Published by Elsevier Ltd. All rights reserved.

Electrochimica Acta

Phosphomolybdic acid nano-aggregates immobilized nafion membrane modified electrode for selective cysteine electrocatalytic oxidation and anti-dermatophytic activity

Journal	Journal of Microbial & Biochemical Technology
Publisher	OMICS Publishing Group
ISSN	19485948
Open Access	0