Raw materials obtained from fish have received significant attention in recent years as an alternative source of collagen as the outbreak of bovine spongiform encephalopathy and foot and mouth disease have led to decline supply of collagen from mammalian sources. Collagen has a wide range of application in pharmaceutical, biomedical and food industries. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from Lagocephalus wheeleri skin were isolated and characterized. The yields of ASC and PSC were 6% and 14% (based on wet weight basis), respectively. According to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns, both ASC and PSC were characterized to type I collagen. The results of Fourier transform infrared (FTIR) spectroscopy of both ASC and PSC were found to be similar. The denaturation temperature (Td) of ASC and PSC were 32.9 °C and 31.9 °C, respectively which was about 5 °C lower than that of calf skin collagen (37 °C). Both collagen exhibited high solubility in acidic pH and there was decrease in solubility of collagens with increase in NaCl concentrations. Hence, the extraction of collagen from L. wheeleri skin could be a favorable way to obtain the value-added products.