In this present study total six bacterial isolates were obtained from paper mill effluent collected from Erode district, Tamilnadu, India. Out of these, two isolates VITNJ12 and VITNJ13 has shown the resistance to heavy metals at maximum concentration of 300mg/ml. Then, the isolates were further characterized by morphological, biochemical and molecular technique and the isolates were found to be Acinetobacterschindeleri and Lysinibacillussphaericus. Both the isolates have shown the maximum resistance to antibiotics such as Rifampicin, Miconazole, Fluconazole, Ceflazidime and Methicillin. The heavy metal and antibiotic resistant bacteria isolated from paper mill effluent could be further explored for bioremediation purpose. © 2015 By IYPF All rights reserved.