The effective utilization of iron by plants for proper growth and yield has been a limiting factor since iron in nature is mostly found in complex form usually as insoluble precipitates that are part of hydroxide polymers. Many plants and micro-organisms produce siderophore for efficient iron transport mechanism. Siderophores particularly are low molecular mass molecules that have high specificity for chelating iron and are able to solubilise and transport ferric ion in the environment, thus making iron available for its frequent uptake by micro-organisms and plant. For the present study the Rhizosphere bacteria of Opuntia sp., which is a drought plant was selected. The bacterial isolates from the rhizo-sphere soil was collected and were screened for its ability to produce siderophore. This study revealed that the Rhizosphere-bacterial population of Opuntia sp. have been found to produce high concentration of siderophore, with catechol-type dominating among the other types. This was identified using CAS media due to formation of Blue colour halozones were formed around the well inoculated. Three species out of five which were isolated were found to be catechol. Then characterization was done by Arnow's Assay, which gave the peak on colorimeter at 480nm for the siderophores producing bacteria and it was quantified using the Desferal Standards, which showed considerable range presence to siderophores in the various supernatant of various samples. © Global Science Publications.