The presence of sulphur in natural form is generally found in acid sulphate soils and in iron bearing sulphides regions. They are also found in effluents released from industries due to anthropogenic activity. The acid manufacturing industries release a huge amount of sulphates in soil that in turn affects the natural habitat of soil. Physical and chemical methods of sulphate removal are highly stereotyped such as lime precipitation, barium precipitation and cost-effective sulphate removal process. Nonetheless, not much has been studied about sulphate removal with microorganisms. This study deals with removal of sulphate with Aspergillus neoflavipes strain AJR1 which was isolated from acid industry effluent rich soil; sulphate tolerance was also checked. The growth kinetics rate of fungus isolate was detected in presence of sulphate which showed increase in growth of the fungus in presence of sulphate. The fungus was used for removal of sulphate from aqueous solution and the removal was analytically checked by high performance liquid chromatography (HPLC). © 2019 E-flow PDF World Research Association. All rights reserved.