The present study aims at isolation of pimple causing bacteria mainly Staphylococcus aureus and P. acnes and to test the antimicrobial action of various toothpastes including Colgate and Dabur on the pathogenic organisms by identifying the microbial inhibition zones and using the minimal inhibitory concentration of toothpastes. Also, further microbial action of some natural substances like Neem,Aloevera was observed on pathogens to determine the best combination of natural and chemical substances effective against the bacteria. Since, there are a lot of products available in market which promise to provide the effective cure against this very common skin lesion "Acne" but every time not effectively do it. By this experiment the main aim which arises is to get a proper combination and inhibitory concentrations of some chemical substances like Triclosan and natural substances like honey and citrus to get an effective solution against these bacteria.