An investigation was conducted to evaluate the potential actinomycetes producing bioactive compounds amended in Amirthi forest, Vellore, Tamil Nadu, India. In this present study 5 soil samples were collected from Amirthi forest range for the isolation of actinomycetes. About 31 strains of actinomycetes were isolated and selected based on their morphological features. The selected isolates were allowed to screen for the antagonistic activity by cross streak method. Out of which one showed potency and the crude extract was subjected for antibiogram at the concentration of 20mg/ml against clinical pathogens. The maximum activity was found against Pseudomonas aeruginosa (20mm).Simultaneously the crude extract was also evaluated for antioxidant activity. The maximum inhibition was found at 5.0mg/ml concentration with 90.7%. Considering the outcome of the present investigation with both antibacerial and antioxidant activity. Identification of the species was performed following molecular taxonomy by 16S rRNA sequencing and found to be Streptomyces sp VITNSJ2.This reports that the unique soil actinomycetes serve as the source to explore bioactive secondary metabolites from forest sources.