The people of South India are often suffering from food poisoning and other health diseases. The isolation of pathogens from domestic refrigerators was performed to determine the prevalence of pathogenic microorganisms. Samples were obtained from domestic refrigerators of various parts of South India (Vellore district). The swabs were inoculated onto Mannitol salt agar, EMB agar, Mac conkey agar and were incubated for 24 hours at 37°C. After incubation 10 bacterial cultures were obtained using various cultivation medium. Gram's staining revealed 10 isolates were Gram negative. For the Gram negative isolates biochemical tests were performed. Catalase test showed positive results for all the isolates. From the morphological and biochemical characteristics the isolates were identified as Salmonella sp., Citrobactor sp., Proteus sp., E. coli. These findings underline the need for greater consumer's education regarding proper cleaning of their refrigerators and safe food handling practices.