This paper concentrates on multi-row machine layout problems that can be accurately formulated as quadratic assignment problems (QAPs). A genetic algorithm-based local search approach is proposed for solving QAPs. In the proposed algorithm, three different mutation operators namely adjacent, pair-wise and insertion or sliding operators are separately combined with a local search method to form a mutation cycle. Effectiveness of introducing the mutation cycle in place of mutation is studied. Performance of the proposed iterated approach is analyzed and the solution qualities obtained are reported. © 2007 Elsevier Ltd. All rights reserved.