The phenomenal growth that has been evidently seen in the last two decades has brought a multifold development in the process industry. The experts have always focused upon the control of the level and other process parameters. In this paper we have chosen a real time Single Spherical Tank Liquid Level System (SSTLLS) for our investigation. The aim of this paper is to compare the design and implementation of Ziegler-Nichols (ZN) and Skogestad’s tuning based PI controllers for the SSTLLS using LabVIEW and NI-DAQmx 6211 data acquisition card. The real time model identification and Graphical User Interface (GUI) are discussed and their PI Controller implementation using LabVIEW are outlined. © 2005 - 2014 JATIT & LLS. All rights reserved.