In the present investigation, ZnO NPs were green synthesized using Lagenaria siceraria Pulp extract. The physicochemical properties of ZnO NPs were characterized using UV-Visible spectro-photometer, X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM) analysis. Further, the biosynthesized ZnO Nanoparticles have been used for evaluating anti-dandruff, anti-microbial and anti-arthritic efficacy. Anti-dandruff activity was assessed against Malassezia furfur MTCC 1765 and Malassezia pachydermatis MTCC No. 1369 and anti-microbial activity was assessed against Klebsiella pneumoniae MTCC No. 9401, Escherichia coli MTCC No. 1691, Staphylococcus aureus MTCC. No. 9542, Listeria monocytogenes MTCC No. 657 and Salmonella sp MTCC No. 1169. In anti-arthritic activity, the percentage stabilization of aqueous extract was found to be 82% and ZnO NPs were found to be 89 % inhibition on BSA method which confirms the anti-arthritic activity. Finally, our results proved that green synthesized ZnO NPs using L. siceraria have a great potential in biomedical applications.