Radial error measurement of a high speed spindle is commonly affected by speed variations, centring error, form error of master cylinder and thermal drift of the spindle. This paper presents a least square curve fitting technique for processing the high speed spindle data using a mathematical model consisting of a second-order polynomial and sum of sinusoidal functions. Re-sampling method is proposed for accounting the spindle speed variations. Performance of the proposed method is compared with Fourier transform based frequency domain filtering method. Simulation and experimental results confirm the accuracy of the proposed method for analysing the time sampled spindle data. Copyright © 2011 Inderscience Enterprises Ltd.