Background: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS19 isolated from agricultural lands in Kolathur, India. Methods: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors–maltose (C-source), malt extract (Nsource), pH, and CaCl2 was studied. Results: The factors pH and CaCl2 concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production. Conclusion: Amylase production was thus optimized and increased yield was achieved. © 2018, Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature.

Subathradevi C

Department of Biotechnology

School of Bio Sciences and Technology

Vellore Campus

Kizhakedathil M.P.J

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Frontiers in Biology

Background: Rhizosphere is rich in microbial flora. Rhizosphere soils samples were collected from five different agricultural fields located in the Kolathur village, Tamil nadu, India. Thirty isolates were isolated and designated as VITMS1-VITMS30. The isolates are checked for the plant growth promotion property. Methods: Plant growth promotion studies such as phosphate solubilization, HCN, IAA, Siderophore and Ammonia production was performed. The potent isolates were assessed for their plant growth promotion using mustard pants. Results: Out of 30 isolates tested, all the 30 isolates showed the ability to solubilize phosphate, produce indole-3 acetic acid (IAA), siderophore and ammonia. HCN production was shown by 11 isolates. Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth. Optimization of Indole acetic acid production was carried out by using Streptomyces sp. VITMS22 at different cultural conditions of pH and temperature with varying media components such as carbon and nitrogen source, tryptophan concentration. The production of IAA was maximum at pH 7.3 and at 28 °C at 0.2% tryptophan when dextrose and peptone were amended as carbon and nitrogen sources respectively. Partial purification of IAA was done and purity was confirmed with thin layer chromatography. Subsequently, effect on plant growth was tested and was proved significant. Genotypic characterization was done using 16S r‑DNA partial gene amplification and sequencing. The sequence was submitted to GenBank with the accession number MF164146. Conclusion: From this experiment, the plant growth promotion on mustard plants was achieved. © 2018

Biocatalysis and Agricultural Biotechnology

Rhizoshpheric bacteria isolated from the agricultural fields of Kolathur, Tamilnadu promotes plant growth in mustard plants

Background: Rhizosphere is rich in microbial flora. Actinomycetes are a diverse group of organisms which are reported to produce antibiotics, vitamins, enzymes and plant growth promoting substances. Aim: The main objective of this study was to screen actinomycetes from the soil samples for the production of various enzymes that could be used for industrial applications. Method: Rhizosphere soils samples were collected from five different agricultural fields located in the Kolathur village, Tamil nadu, India. Thirty isolates were isolated and designated as VITMS1-VITMS30. The maximum number of isolates (43.3 %) was obtained from the rhizosphere soil samples of brinjal field and maximum number of isolates was isolated on starch casein agar (90%). The isolates were characterized morphologically in ISP media 1-7, Starch casein agar, Bennet agar and AIA, respectively. Results: The isolates were screened for eight different enzymes – α-amylase, protease, gelatinase, chitinase, lipase, DNase, cellulase and urease. From the results observed, all the thirty isolates showed positive α-amylase, lipase and gelatinase activity. All the isolates exhibited cellulase activity but in very low levels. Out of 30 isolates, 19 isolates exhibited protease activity; 24 isolates were positive for DNase activity; 10 isolates showed chitinase activity and 20 isolates showed urease activity. Conclusion: The soil samples from the kolathur village harbor diverse actinomycetes which exhibited good enzymatic activity. © 2018 Bentham Science Publishers.

Current Bioactive Compounds

Screening for Extracellular Enzymes from Actinomycetes Isolated from Agricultural Soils of Kolathur, Tamil Nadu, India

Bacteriocins have been produced by various Lactic acid bacteria (LAB) strains isolated from dairy and fermented vegetable sources. In the current study we have isolated a novel bacteriocin producing strain Pediococcus pentosaceus KC692718 from mixed vegetable pickles (India). A 2 step process optimization for enhancing production of bacteriocin from the isolates was carried out with One-factor-at-a-time (OFAT) and Response Surface Methodology (RSM) methods. A 2.5 fold (AU/mL) increase of bacteriocin was observed for sucrose (2.4 %) as carbon source and 4.7 fold (AU/mL) increased bacteriocin was observed in the presence of soyatone (1.03 %) as nitrogen source in the OFAT experiments. In order to increase bacteriocin production RSM tool was performed with optimized chemical and physical sources using Design expert 8.0.7.1. Soyatone (1.03 %), sucrose (2.4 %), pH (5.5) and temperature (34.5 ºC) condition yielded 25,600.34 AU/mL of bacteriocin from P. pentosaceus KC692718. This is the first report which has produced 20 fold increase of bacteriocin for Pediococcus pentosaceus KC692718 from that of MRS medium with 1 280 AU/mL © 2014, Association of Food Scientists &amp; Technologists (India).

Fulltext

Journal of Food Science and Technology

Optimization studies for enhanced bacteriocin production by Pediococcus pentosaceus KC692718 using response surface methodology

Brazilian Archives of Biology and Technology

Optimization of the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 by response surface methodology

International Journal of Microbiology

Optimization of Amylase Production fromB. amyloliquefaciens(MTCC 1270) Using Solid State Fermentation

BMC Microbiology

Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts

Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology

Journal	Data powered by TypesetFrontiers in Biology
Publisher	Data powered by TypesetSpringer Science and Business Media LLC
ISSN	1674-7984
Open Access	0