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Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology
Kizhakedathil M.P.J,
Published in Springer Science and Business Media LLC
Volume: 13
Issue: 2
Pages: 123 - 129
Background: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS19 isolated from agricultural lands in Kolathur, India. Methods: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors–maltose (C-source), malt extract (Nsource), pH, and CaCl2 was studied. Results: The factors pH and CaCl2 concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production. Conclusion: Amylase production was thus optimized and increased yield was achieved. © 2018, Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature.
About the journal
JournalData powered by TypesetFrontiers in Biology
PublisherData powered by TypesetSpringer Science and Business Media LLC
Open Access0