Melioidosis caused by Burkholderia pseudomallei (B. pseudomallei), is a predominant infectious disease in tropical countries. Melioidosis awareness is surprisingly low among physicians and health care workers in endemic regions in spite of its higher prevalence. In recent decades, there is a significant interest in comparing the B. pseudomallei genomes of different strains as there is a rapid increase in the number of whole genome sequences from B. pseudomallei strains. Thus, it is imperative to provide organism specific annotations to the scientific community exploring on B. pseudomallei. This prompted us to develop a database exclusively for melioidosis and thus, we have developed the "Melioidosis database" (MDB). Various tools have been deployed in MDB to compare gene and protein sequences of B. pseudomallei strains. The MDB aids in the analysis of phenotypic variations also to perform comprehensive proteomics and genomics studies. The database can be accessed at http://bioserver1.physics.iisc.ernet.in/MDB/ and it will be updated regularly. © 2017, Research Journal of BioTechnology. All rights reserved.