The changes induced in bacterial strains under stress conditions provide an insight into metal resistance strategies. Trivalent chromium resistant bacterium were isolated and identified by 16S rRNA gene sequencing and designated as Alcaligenes faecalis VITSIM2. The growth pattern was monitored. The organism also showed resistance to copper, cadmium, and certain antibiotics. The differentially expressed proteins in SDS PAGE were identified by mass spectrometry as flagellin and 50S ribosomal L36 protein. The morphological changes were identified by scanning electron microscopy. The changes in the cell wall content were estimated by peptidoglycan analysis and transformation of phosphates was detected by 31P NMR. Flow cytometry was employed to measure the membrane integrity, esterase activity and intracellular pH. In conclusion spectrum of proteomic, physiological, and morphological alterations was observed that aid the organism to overcome chromium stress. © 2017 WILEY-VCH Verlag GmbH &amp; Co. KGaA, Weinheim

Shanthi C

Department of Biotechnology

School of Bio Sciences and Technology

Vellore Campus

Matilda S.C

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Basic Microbiology

Aims: The aim of the study was to investigate the proteomic changes and antioxidant enzyme activity in chromium-resistant Bacillus cereus VITSH1 in response to heavy metal toxicity. Methods and Results: The variation in protein expression and antioxidant enzyme activity in the presence and absence of metal was studied in B. cereus VITSH1. The differentially expressed proteins in chromium-treated conditions were determined by SDS PAGE. The proteins involved in metal binding, protein biosynthesis, protein folding, energy metabolism and motility were identified by mass spectrometry coupled with bioinformatics search tools. The in gel assays for antioxidant enzymes indicated a change in their activity under metal stress conditions. Conclusions: The findings of this study suggest that the organism combats metal stress probably by restricting the entry of metal inside the cell. The role of the differentially expressed proteins clearly indicates that the first line of defence is to avoid the entry of metal into the cell either by possessing a modified outer membrane or by moving away from the toxicant. Further work on the identification of other proteins playing a role in resistance would help in integrating the available knowledge on the resistance mechanisms the organisms employ to combat toxicity. Significance and Impact of the Study: The proteomic changes in the metal-exposed bacteria would give an insight into the proteins involved in metal resistance mechanisms and thereby aid in the development of biosensor-based technology for heavy metal detection. © 2019 The Society for Applied Microbiology

Journal of Applied Microbiology

Protein profiling of metal‐resistant Bacillus cereus VITSH1

Metal induced changes in trivalent chromium resistantAlcaligenes faecalisVITSIM2

Potential of proteomics to probe microbes

Journal of Chromatography B

Affinity selection of histidine-containing peptides using metal chelate methacrylate monolithic disk for targeted LC–MS/MS approach in high-throughput proteomics

BMC Genomics

Co-occurrence of resistance genes to antibiotics, biocides and metals reveals novel insights into their co-selection potential

Geomicrobiology Journal

Isolation and Molecular Characterization of Heavy Metal-ResistantAlcaligenes faecalisfrom Sewage Wastewater and Synthesis of Silver Nanoparticles

Extremophiles

Inorganic polyphosphates in extremophiles and their possible functions

Journal	Data powered by TypesetJournal of Basic Microbiology
Publisher	Data powered by TypesetWiley
ISSN	0233-111X
Open Access	0