The aim of the study was to investigate antimicrobial activity, minimal inhibitory concentration of samples such as tulsi, turmeric, olive oil, aloevera gel and peel, neem, saffron, green camphor, amla, rose petals, sandals, bengal gram coconut oil was collected from various places for biocosmetics preparation. Dimethyl sulfoxide were used to screen antimicrobial activity by agar diffusion method. The higher antibacterial activities were observed in ethanolic extract followed by and di-Methyl sulfoxide. Aqueous ethanolic extract of four medicinal plants were subjected to antibacterial assay against human pathogenic Staphylococcus aureus, determination of minimal inhibitory concentration [MIC] for every chemical agent through the dilution method. Immobilization technique is used for preservation of enzymes using different colors of natural dyes for the importance would be to create beads of different colors. Results of antimicrobial activity was seen maximum in coconut oil and MIC of various samples will be discussed. © 2015, Sphinx Knowledge House. All rights reserved.