This paper proposes the application of a novel time-frequency analysis (TFA) technique, the S-transform (ST) on spinal somatosensory evoked potentials (SSEP). Variations of ST were used to analyze SSEP signals for monitoring the integrity of spinal cord during spinal surgery. A graphical user interface (GUI) was developed in MATLAB® to analyze SSEP signals using a few types of ST. Analysis had been carried out to compare the usefulness of generalized S-transform (GST), complex S-transform (CST) and hyperbolic S-transform (HST) with short-time Fourier transform (STFT). Time-frequency features were extracted from the ST plot of SSEP signals to determine the usefulness of the proposed techniques. It is observed from the analysis that GST, CST and HST indeed provide better results compared to STFT.