A transparent oil-in-water nanoemulsion system consisting of eucalyptus oil, Tween-20 as organic phase and water as an aqueous phase was developed using a low energy emulsification method. Physicochemical properties such as droplet size, optical transparency, and long-term stability was studied. The stable eucalyptus oil nanoemulsion (1:2) having mean droplet size in the range of 50-100 nm with a polydispersity index &lt;0.2. The optimized nanoemulsion formulation exhibited significantly higher antibacterial activity by the well diffusion method against Listeria monocytogenes. Further, alteration in the membrane integrity was assessed, and it is found higher for nanoemulsion treated cells than control cells. Atomic force microscopic observations showed distorted morphology of treated bacterial cells. These results propose the possible use of eucalyptus oil nanoemulsion in the food industries. © 2015, Medknow Publications. All rights reserved.

Amitava Mukherjee

Centre for Nanobiotechnology

Vellore Campus

Chandra Sekaran N

Sugumar S

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Asian Journal of Pharmaceutics

Environmental Chemistry Letters

Nanoemulsion ingredients and components

The current study deals with the formulation and characterization of bio-based oil in water nanoemulsion and its potential antibacterial activity. A typical v/v% of eucalyptus oil (16.66%), Tween 80 (16.66%), and water (68.68%) was prepared by ultrasonication method. The mean droplet size was 17.1nm as confirmed by dynamic light scattering. Different concentrations of the formulation ranging from undiluted to 10-, 100-, and 1000-fold dilutions were used to check the antibacterial activity in three different microorganisms, namely, Bacillus cereus, Staphylococcus aureus (Gram-positive), and Escherichia coli (Gram-negative). All three species showed a 100% bactericidal at the 10-fold dilution of the nanoemulsion formulation in the following order: B. cereus at 0th min, S. aureus at 15min and E. coli at 1h, respectively. A 10-fold dilution of the nanoemulsion showed that, the cytoplasmic content leakage from the bacterial species was high for S. aureus when compared to B. cereus and E. coli as determined by UV-Vis spectroscopic method. Fluorescence microscopic technique further confirmed this study. © 2013.

Journal of Basic Microbiology

Bio-based nanoemulsion formulation, characterization and antibacterial activity against food-borne pathogens

PVT Property Correlations

Copyright

Food Chemistry

Vitamin E bioaccessibility: Influence of carrier oil type on digestion and release of emulsified α-tocopherol acetate

Nanoemulsion delivery systems: Influence of carrier oil on β-carotene bioaccessibility

Soft Matter

Nanoemulsions versus microemulsions: terminology, differences, and similarities

Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes

Journal	Asian Journal of Pharmaceutics
Publisher	BRNSS Publication Hub
ISSN	0973-8398
Open Access	0