In recent years, nanotechnology-based studies have been employed in the area of systems biology. The current chapter aims to give a concise view of this emergent field of research, namely nano systems biology. A large number of such studies are based on understanding surface reactivities of a biological system. Another stream of studies is focused on imaging approaches using nano systems biology. In this chapter, the authors also illustrate state-of-the-art work using these approaches in nanomedicine.