Electrochemical glucose sensor was fabricated using graphene/CuO nanocomposites, which was prepared by electrodeposition method, i.e., copper oxide on graphene nanosheets immobilized on the glassy carbon electrode (GCE) using Nafion (Nf) as binder. The graphene oxide sheets were obtained by chemical functionalization and exfoliation of graphite using modified Hummer's method. Electrodeposition proved to be an elegant route to coat the electroactive copper oxide over the carbonaceous sheets. The CuO coated graphene serve as a promising electrode material for non-enzymatic glucose sensor. The morphological and electrochemical response of glucose was done using field-emission scanning electron microscope (FE-SEM) and electrochemical workstation. Compared with other sensors, the proposed sensor shows high sensitivity, good reproducibility, a fast amperometric response and good selectivity. As a result the proposed material exhibited a linear amperometric response range of glucose 10 μM to 90 μM at an oxidation potential of 0.6 V versus Ag/AgCl; with a sensitivity of 86 μA/mM (1211.3 μAmM-1cm-2). Copyright © 2017 American Scientific Publishers All rights reserved.