The heat transfer in nanofluids plays a major role in solar energy, nuclear reactors, aerodynamics, etc. By keeping this in view, in this study, we investigated the flow and heat transfer nature of liquid film flow of ethylene glycol (EG)-Cu nanofluid in the presence of non-uniform heat source/sink. We considered the Jeffrey fluid model to investigate the flow and heat transfer behavior. The governing equations are transformed as ordinary differential equations with the aid of similarity variables. Numerical results are carried out by employing bvp5c Matlab package. The influence of pertinent parameters on velocity and temperature profiles along with the reduced Nusselt number is discussed with the help of graphs and tabular results. It is observed that the rising value of the non-uniform heat source/sink parameter acts like heat generators and regulates the thermal field. Rising the film thickness enhances the heat transfer rate.