The pathogens which are the effective transmitters of various infections pose a serious problem in restraining their interference in maintaining a sterile environment. The practical applicability of traditional methods of disinfection is restricted due to their cumbersomeness, toxic product generation, and cost-effectiveness. Therefore, the objective of the current review is to elaborate the efficacies and limitations of various novel disinfectants that can show their activity in a few minutes of treatment. The expected outcome would be feasibility for selection of a favorable disinfectant through various technologies that can generate uniform results and form a basis for the true estimation required parameters. Hence, the current paper ends with the consideration of unique new techniques that distinguishes their simplicity, safety, and efficacy in generating a sterile environment. © Carl Hanser Publisher, Munich.