Abstract Herbal medicines play an important role in healthcare sector all through the world. Herbal medications are presently broadly been used as a strong solution for the counteraction and management of multiple health conditions. The prime method of transmission of microorganisms and nosocomial diseases is through our hands. Thus, hand-washing is an essential practice in human services and household part. Various antiseptic hand wash available in the market are alcohol based sanitizers which have been reported to show some adverse effects on our soft hands. To keep away from these unfriendly impacts like itching, drying, irritation, dermatitis etc., of the synthetic hand wash formulations an attempt has been made to formulate a poly herbal hand wash. The current exploration was planned to assess the antibacterial viability of different spices and fruits such as coriander, cumin, orange, walnut, curry leaves, mint, ginger, lemon grass, rosemary, aloe Vera. Examination was done to detail and assess the polyherbal powder hand wash. The anti-microbial activity of the formulated herbal hand wash powder was tested against Escherichia coli and Staphylococcus aureus isolated from cosmetics by pour plate procedure and MIC tally. Likewise the effectiveness was checked by utilizing the hand wash powder on volunteers.