Sivakumar S

Department of Social Sciences

School of Social Sciences & Languages

Vellore Campus

Rajkumar T

Taju G

Abdul Majeed S

Sinwan Sajid M

Santhosh Kumar S

Thamizhvanan S

Vimal S

Sahul Hameed A.S.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Developmental & Comparative Immunology

The gene encoding VP28 protein of WSSV was cloned into pRSET B vector, and recombinant VP28 (r-VP28) protein was produced in E. coli GJ1158 by induction with NaCl. The r-VP28 protein was purified. The purified r-VP28 protein was injected intramuscularly in Whiteleg shrimp, Litopenaeus vannamei, to study its tissue distribution at different time intervals by ELISA using the antiserum raised against r-VP28, and the results showed the presence of r-VP28 protein in different organs such as heart, hepatopancreas, gill, muscle, and hemolymph. The r-VP28 protein was cleared from all the organs after 264Â h post-injection (hpi). Various immunological parameters such as phenoloxidase activity (PO), superoxide anion production, activity of superoxide dismutase, and MDA content were assessed in normal and experimentally r-VP28 protein injected shrimp at different time intervals. The mRNA expression of ten immune-related genes was analyzed in hemocytes, hepatopancreas, and gill by quantitative real-time PCR in order to investigate their expression to r-VP28 injection in L. vannamei, and the results showed the upregulation of expression of immune genes such as PO, cMnSOD, lipopolysaccharides (LPs), BGBP, hemocyanin, crustin, lectin, toll receptor, lysozyme, and tumor necrosis factor receptor-associated factor 6 (TRAF6) in VP28-injected shrimp. The efficacy study revealed that 86.67% survival was observed in shrimp orally treated with r-VP28 protein after WSSV challenge without any clinical sign of WSSV infection till the end of experimental period.

Aquaculture International

Distribution of recombinant VP28 protein in tissues and its immunomodulatory effect against white spot syndrome virus in whiteleg shrimp, Litopenaeus vannamei (Boone, 1931)

Aquaculture Research

Immunomodulatory effect ofCynodon dactylonagainst white tail disease of giant freshwater prawn,Macrobrachium rosenbergii(de Man, 1879)

Antiviral Research

Expression profile of bio-defense genes in Penaeus monodon gills in response to formalin inactivated white spot syndrome virus vaccine

Aquaculture

Protective effect of microbial immunostimulants and antiviral plants against WSSV in Litopenaeus vannamei cultured under laboratory conditions

Fish & Shellfish Immunology

Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei

Ontogenetic changes in the expression of immune related genes in response to immunostimulants and resistance against white spot syndrome virus in Litopenaeus vannamei

Journal	Data powered by TypesetDevelopmental & Comparative Immunology
Publisher	Data powered by TypesetElsevier BV
ISSN	0145-305X
Open Access	0