A newly isolated alkalophilic, thermophilic Bacillus RV.B2.90 was characterized and tested for the production of extracellular protease in 50 - 300 ml shake flask experiments. The strain was identified as Bacillus cereus by biochemical and 16S rRNA sequencing. Maximum amount of enzyme produced in basal medium at exponential phase was 1284 U/ml. The optimized medium could increase enzyme production by 30% to 4189 U/ml. Growth and enzyme production occurred in media at all initial pH ranging from 7.0 - 12.0, the maximum enzyme production occurred in the medium with initial pH 8.0. The pH increased with growth. Depending on the starting pH 7.0 - 12.0 the duration of incubation required for change of pH to 9.0-10.0 varied markedly 40 - 12 hrs. This phase coincided with maximal protease production at each pH. Production of enzyme increased significantly with temperature of incubation from 37-70°C, with 1284 U/ml at 37°C and 1984 U/ml at 70°C. At 90°C the growth and enzyme production showed a steep decline to 400 U/ml. With respect to other growth parameters, the optimum agitation speed was 50 rpm, the best carbon sources were xylose and arabinose that enhanced enzyme production to 4189 U/ml and 4016 U/ml, the best nitrogen source were casein and malt extract showing 3946 U/ml and 3182 U/ml. Inorganic nitrogen sources did not have significant effect on enzyme production. Sodium chloride at 4% (w/v) was found to be optimum for enzyme production. Among the 5 amino acids added to the medium, histidine increased enzyme production to 1853 U/ml (about 30%). Among the salts added to the medium K +, Mg 2+ and Ca2+ were found to increase the enzyme production. The isolate was found to be highly alkalophilic, thermophilic, thermo stable and moderately halophilic protease producing organism.