Schematic representation of in vitro cancer therapy using DOX loaded PEGylated FePt/Fe3O4 composite nanoassemblies (CNAs) in the presence of ACMF or H2O2.

N.K. Sahu

Jagriti Srinivasalu Gupta

Sahu N.K

D. Bahadur

Gupta J

Bahadur D.

Cell culture

Cells

chemotherapy

Controlled drug delivery

Diseases

hydrothermal synthesis

Iron oxides

Lanthanum compounds

magnetite

Oxygen

Targeted drug delivery

Tumors

Alternating current

Clinical application

Mouse-fibroblasts

Negatively charged

Reactive oxygen species

Synergistic combinations

Therapeutic drugs

Therapeutic effects

Platinum compounds

antineoplastic agent

doxorubicin

Hydrogen peroxide

Iron

macrogol derivative

nanomaterial

organometallic compound

Platinum

reactive oxygen metabolite

Animal

Cell Line

cell survival

chemistry

dose response

drug delivery system

drug effects

fever

HeLa cell line

Human

metabolism

mouse

structure activity relation

synthesis

Animals

Antineoplastic Agents

Dose-Response Relationship, Drug

Drug Delivery Systems

Ferrosoferric Oxide

HeLa Cells

Humans

Mice

Nanostructures

Organometallic Compounds

Polyethylene glycols

Structure-Activity Relationship

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Dalton Transactions

Magnetite (Fe3O4) nanoparticles (NPs) with various morphologies obtained by varying solvent (water)-to-surfactant (polyethylene glycol, PEG) ratios have been investigated for magnetic hyperthermia (MHT) application. The water-to-PEG ratio influenced the size, shape, and chemical composition of the NPs, which changes their magnetic properties and hyperthermia (HPT) response. X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) studies reveal the formation of well crystalline inverse spinel structure of Fe3O4 with some Fe0 phases. Morphology of the NPs varies from nearly spherical to elongated to pseudohexagonal to cluster with an increased concentration of PEG. The NPs possess enhanced saturation magnetization ranging from 90 to 98&nbsp;emu/g. The HPT studies indicated that the NPs show an enough specific absorption rate (SAR) under alternating magnetic field suitable for biological application with hexagonal Fe3O4 being more efficient. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Journal of Superconductivity and Novel Magnetism

Water-to-PEG Variation: Morphology and Hyperthermic Behaviour of Iron Oxide

Antibacterial activity of nanoparticles (NPs) and nanocomposites (NCs) has received wide spread attention in biomedical applications. In this direction, the authors prepared zinc oxide (ZnO), iron oxide (Fe3O4), and their composite including reduced graphene oxide (rGO) by hydrothermal method. The structural and microstructural properties of the synthesised NPs and NCs were investigated by XRD, FT-IR, UV-Vis, TGA, and TEM analysis. PEG-coated ZnO and Fe3O4 form in hexagonal wurtzite and inverse spinel structures, respectively. ZnO forms in rod-shaped (aspect ratio of ∼3) morphology, whereas well-dispersed spherical-shaped morphology of ∼10 nm is observed in Fe3O4 NPs. The ZnO/Fe3O4 composite possesses a homogeneous distribution of above two phases and shows a very good colloidal stability in aqueous solvent. These synthesised particles exhibited varying antibacterial activity against gram-positive strain Staphylococcus aureus (S. aureus) and gram-negative strain Escherichia coli (E. coli). The nanocomposite exhibits a better cidal effect on E. coli when compared to S. aureus when treated with 1 mg/ml concentration. Further, the addition of rGO has intensified the anti-bacterial effect to a much higher extent due to synergistic influence of individual components. © The Institution of Engineering and Technology 2019.

IET Nanobiotechnology

Synthesis of ZnO/Fe3O4/rGO nanocomposites and evaluation of antibacterial activities towards E. coli and S. aureus

A comparative study has been done on the drug loading efficacy of different surface functionalized Fe3O4 magnetic nanoparticles (MNP) and their effect toward ovarian cancer cell line (HeLa) in vitro. The surface coating was done with carboxyl group enriched organic ligands such as succinic acid, L-arginine, oxalic acid, citric acid and glutamic acid. A maximum of ∼62 wt.% drug has been loaded in oxalic acid coated MNP whereas least (∼35 wt.%) in arginine coated MNP due to variation in the chemistry of surface active agents. The cytotoxicity of functionalized MNP and doxorubicin loaded MNP were studied. The functionalized MNP are quite biocompatible whereas they show cytotoxic effect in HeLa cells e.g., around 35% cell death when treated with oxalic acid coated MNP at a concentration of ∼100 μg/ml. The cell death was triggered in drug loaded particle and almost complete cell death i.e., &gt;90% was observed in oxalic acid coated MNP followed by ∼80% and ∼70% death in arginine and succinic acid coated MNP, respectively. Copyright © 2017 American Scientific Publishers All rights reserved.

Journal of Nanoscience and Nanotechnology

Synthesis of surface grafted mesoporous magnetic nanoparticles for cancer therapy

PEGylated FePt-Fe3O4 composite nanoassemblies (CNAs): In vitro hyperthermia, drug delivery and generation of reactive oxygen species (ROS)

Journal of Alloys and Compounds

A comparative study on the supercapacitive behaviour of solvothermally prepared metal ferrite (MFe2O4, M = Fe, Co, Ni, Mn, Cu, Zn) nanoassemblies

We report the formation mechanism of FePt nanoparticles (NPs) by a high temperature polyol method using an equimolar ratio of Fe and Pt-precursor with different Pt-precursors. Pt(acac)2, PtCl2, PtCl 4 and H2PtCl6·H2O were used as Pt-precursors and Fe(acac)3 as the only Fe-precursor. Different stoichiometric compositions along with variation in size were obtained by using different precursors of Pt. Nearly, equiatomic FePt having a size ∼2 nm was formed with Pt(acac)2. However, Pt rich phases were formed using all other precursors with a size ranging between 3.6 to 6.4 nm. It was found that the atomic percentage (at%) of Fe in the FePt NPs depends on the reaction parameters. The decomposition behaviour of Fe and Pt-precursors were examined by vibrating sample magnetometer and thermogravimetric measurements. A possible reaction mechanism for Fe depleted FePt formation is proposed which suggests that the reduction potential and decomposition behaviour of the organic and inorganic salts of Pt significantly modify the nucleation behaviour. The electrocatalytic properties of all the four nanomaterials towards methanol oxidation have been investigated by cyclic voltammetry. It is found that Fe 19Pt81 with an average size of 6.2 nm shows the highest catalytic response. © 2014 The Royal Society of Chemistry.

Role of different platinum precursors on the formation and reaction mechanism of FePt nanoparticles and their electrocatalytic performance towards methanol oxidation

We report a biphasic system (BPS) consisting of PEGylated Tb 3+-doped GdPO4 nanorice sensitized with Ce3+ (PEG-NRs) and glutamic acid coated iron oxide nanoparticles (IONPs) with multifunctional capabilities. The mesoporous PEG-NRs exhibit green light luminescence properties and a high degree of aqueous stability. Their drug loading and release capacities were investigated for anti-cancer chemo doxorubicin (DOX). Their mesoporous nature and availability of plenty of negatively charged functional groups (-COO-) on the surface of PEG-NRs facilitate approximately 94 wt% DOX loading. In vitro studies carried out for PEG-NRs and their biphasic integrated system with iron oxide using HeLa and MCF-7 cell lines demonstrated their cell killing efficacy. The green luminescence observed under confocal laser scanning microscopy (CLSM) confirms the cellular uptake of PEG-NRs by HeLa cell lines and their accumulation in the cytoplasm. Approximately 50-55% of HeLa and MCF-7 cell death was observed after 24 h of incubation with DOX loaded BPS (2 mg IONPs and 0.25 mg PEG-NRs + DOX), which further increased to about 90% when exposed to an AC magnetic field (ACMF) for 25 min. Our findings demonstrate that the therapeutic efficacy of BPS loaded with DOX could be a powerful multimodal system for imaging and synergistic chemo-thermal cancer therapy. This journal is © the Partner Organisations 2014.

Ce3+ sensitized GdPO4:Tb3+ with iron oxide nanoparticles: A potential biphasic system for cancer theranostics

Biomater. Sci.

Biocompatibility, biodistribution and efficacy of magnetic nanohydrogels in inhibiting growth of tumors in experimental mice models

Herein, we report magnetic properties of as-synthesized face centered cubic (fcc)-FePt nanoparticles (∼5 nm) and its transformed face centered tetragonal (fct) phase when annealed at 600°C. We observe weak ferromagnetic nature in fcc phase with non-saturating M-H loop indicating the presence of a large fraction of superparamagnetic particles. Excess of Fe-precursor with respect to Pt used in the reaction accumulates on the surface of the FePt nanoparticles and forms a thin layer of Fe-byproduct (Fe3O 4) which leads to the formation of FePt/Fe3O4 core/shell structure. This was confirmed by X-ray diffraction, X-ray photoelectron spectroscopy, and microstructural studies. Interestingly due to core/shell formation, the coercivity (Hc) is higher at 300 K than at 50 and 100 K, but there is a steep increase at 5 K. Moreover, hysteresis loop is regular at 300 K whereas it is distorted at 5 K. The reason for temperature dependent Hc anomaly and distortion in M-H loop was examined systematically. The competing exchange interaction seems to be responsible for changes in the magnetic behavior. Besides this, it shows spin glass like behavior much below the blocking temperature which is supposed to be due to existence of two magnetic phases and the exchange coupling between core and shell. This has been confirmed from temperature dependent dc magnetization and ac susceptibility studies. We establish a clear correlation between the magnetization dynamics and the core/shell structure of the nanoparticles. On the other hand, the fct phase shows a very large coercivity with some irregularity in the M-H loop. This irregularity may be ascribed due to segregation of fcc-Fe3Pt (soft) phase on the grain boundaries of fct-FePt (hard). Such inhomogeneity in bimagnetic systems (soft-soft or soft-hard) has strong influence on the nanomagnetism. © 2013 American Institute of Physics.

Journal of Applied Physics

Influence of excess Fe accumulation over the surface of FePt nanoparticles: Structural and magnetic properties

Journal	Data powered by TypesetDalton Transactions
Publisher	Data powered by TypesetRoyal Society of Chemistry
ISSN	14779226
Open Access	Yes