Dimensional incompatibility between rider and motorcycle is one of the most causative factors responsible for the prevalence of musculoskeletal discomfort among motorcyclists. The present study aims to identify the comfortable riding posture (CRP) and optimum riding position (ORP) for improving motorcycle design for better riding experience. The data (through image processing technique) was acquired from 120 Indian male motorcyclists (aged 19–40 years) using a static simulator test-rig. The CRP was achieved by adjusting three controls (handgrip, seat, and footrest of the test-rig), and perceived comfort and discomfort ratings. Weighed mean comfort joint angles of ten body-joints defining CRP were estimated and compared with the earlier studies. The best possible ORP among the nine test conditions (defined by the positions of the three controls) was estimated using Taguchi DOE. The study outcomes will help automobile designers to conceptualize the comfortable standard motorcycles. © 2021 Elsevier B.V.