The focus of this investigation is on biodiesel produced from juliflora seeds using the 2-stage acid transesterification process followed by alkali transesterification process producing 80% yield of Juliflra Oil Methyl Ester. Experiments were conducted on single cylinder diesel engine using juliflora biodiesel and its diesel blends. The experimental results of fuel blends (B20, B30, B40, and B100) were compared with those from diesel (D100). The results indicated the performance and combustion characteristics of B20 as almost in line with those of diesel fuel trend. Brake Specific Fuel Consumption (BSFC) for blends B20 and B30 (0.27kg/KWh) at full load was closer to diesel (0.26 kg/KWh). The BTE for Juliflora Biodiesel B100 is 31.11% and it was closer to diesel (32.05%) at full load. However the emission characteristics of CO, HC and smoke for biodiesel and its blends were smaller or equal compared to diesel throughout the experiment. At full load, the NOx for biodiesel B100 was 1832 ppm which was a little higher than diesel fuel (1821 ppm). This led to the conclusion of B20 being most suitable blend of other blends and it is substitute of diesel which will reduce diesel consumption by 20%. © 2019 Elsevier Ltd