In the present study, bioactivity of Emblica officinalis and Phyllanthus emblica leaves and fruit extract with methanol as the solvent was evaluated. Antimicrobial activity against nine clinical pathogens, viz. Staphylococcus aureus, Escherichia coli, Enterococcus sp., Proteus mirabilis, Shigella dysenteriae, Salmonella sp., Klebsiella pneumoniae, Serratia marcescens and Pseudomonas aeruginosa was determined. The methanol extraction of Emblica officinalis and Phyllanthus emblica leaves and fruits were performed using Soxhlet apparatus. The antimicrobial analysis showed that the extracts were more effective against Gram negative bacteria than Gram positive bacteria. Antioxidant activity was performed by DPPH assay followed by cytotoxicity against MG63 osteosarcoma cell lines which was found to be profoundly significant. The leaf extract of both Emblica officinalis and Phyllanthus emblica was proved to be more effective than the leaf extract. The extracts were analyzed through thin layer chromatography (TLC) and maximum Rf value was observed with chloroform:ethyl acetate: acetic acid. GC-MS analysis of extracts was also done to identify the biologically active compounds. Molecular docking studies were performed for the small amla extract compound against bacterial topoisomerases to validate the in vitro results.