Marine environmental conditions are extremely different from terrestrial ones, because every class of marine microbes produces a number of bioactive compounds with unique structural features. Of all the marine microbes, the actinomycetes have a special consideration since it produces a novel bioactive compounds. Hence, marine sediments, collected from the coastal areas of Rameshwaram and Dhanushkodi of Tamilnadu state, India, were screened and eighteen isolates were obtained on Starch-Casein agar media by soil dilution technique. However, only seven isolates showed significant antibacterial activity against Escherichia coli, Proteus mirabilis, Bacillus cereus, and Staphylococcous aureus. GC-MS results reveal that two compounds N-Isopropyluredo Acetic Acid and Benzene Propanamine, N-(1, 1-Dimethyl Ethyl)-Alpha Methyl-Gamma Phosphate were present in the isolates. Morphological, cultural, biochemical characterization and Gene sequencing indicated that the isolates belong to Streptomyces genus of Actinomycetes. The Phylogenetic study reveals the species name as Steptomyces globosus, and hence the strain is named as VITR004. Therefore, the isolation, characterization of the rare actinomycetes from the coastal areas of Rameshwaram and Dhanushkodi of Tamilnadu state, India will be useful for the discovery of the novel bioactive metabolites that are effective against wide range of pathogens.