Suresh P-K

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

&nbsp;

&nbsp;

VIT University

Trends in Biotechnology

Models of tumor heterogeneity should mimic perfusion in the tumor mass and consider cancer stem cell hierarchy and convertibility. Other important factors include epigenomic alterations, enhanced permeation and retention effects, and the reticuloendothelial system. New stimuli-responsive delivery systems, active targeting-based methods, and corona characterization strategies can deliver genes more precisely. © 2018 Elsevier Ltd

Tumor Heterogeneity: An Important Determinant for Efficacy and Safety in Nanoparticle Anticancer Gene Therapy

A novel, colloidal nanogold-based drug delivery system for phenytoin, a well-known anti-epileptic drug with an enhanced efflux via P-glycoprotein, has been proposed in this study. The vital physical properties that would aid in predicting the biological interaction of this system were profiled using various techniques such as UV-Vis, DLS, and TEM in corroboration with theoretical calculations. It was significant to note that the binding of phenytoin to colloidal nanogold was strongly pH-dependent with the optimum at pH 5.5 and a consistently reproducible spectral shift. Analysis of the conjugate by FTIR revealed that the imide functional group of phenytoin mediated a dative coordinate bond to colloidal nanogold at the optimum pH. The amount of the drug bound to the gold was quantified to be 85.8±2.5% (w/v) by HPLC. Hypothetically, the surface charge of the conjugate could possibly imply charge-mediated uptake across the cell membrane. Further, the novel conjugate was screened for its cytotoxicity in two cell lines and the dosage range was identified. Subsequent development, thorough evaluations in suitable model systems, and the potential for bioimaging to track the payload would validate our hypothesis on the conjugate for better intracellular retention at the site of action, and thereby achieve the targeted delivery. © Suneetha et al.

Fulltext

Scientia Pharmaceutica

Physicochemical Characterization and Cytotoxicity Screening of a Novel Colloidal Nanogold-Based Phenytoin Conjugate

Lipophilicity vs hydrophicility physicochemical traits are extremely important variables that are active considerations for optimizing drug delivery systems. The comparative anti-inflammatory delivery potential of dexamethasone (dex) in an encapsulation-based (liposome-lipophilic) and poly (amidoamine) (PAMAM) dendrimer prodrug conjugation-based delivery systems (hydrophilic) was performed in this work. Dendrimer prodrug conjugates were characterized by 1H NMR. The drug encapsulation efficiency for drug in liposomes was observed to be 14.02% and this was correlated with a dose-dependent tumor necrosis factor (TNF)-α inhibition (39-57% inhibition). The biological evaluation of nanocarriers for drug was demonstrated in a standard, conventionally used in vitro cell-based system for TNF-α inhibition. This served as a comparative tool to demonstrate a quantitatively higher TNF-α inhibition (67-71.48%) produced by the dendrimer-dex drug conjugate. The structure activity relationship (dose-for-dose) was inferred by relatively lesser inhibition of TNF-α by variants of PAMAM G4 (NH2) dendrimer-dex conjugates and was compared with liposomes carrying dex. In vitro results suggest that the prodrug conjugates of PAMAM dendrimer deliver dex to be more efficient in comparison with liposome-based dex in terms of higher TNF-α inhibition. This study has implications in designing efficient prodrug nanocarrier systems for delivering dex. © 2013 Elsevier B.V. All rights reserved.

International Journal of Pharmaceutics

Comparative anti-inflammatory activity of poly(amidoamine) (PAMAM) dendrimer–dexamethasone conjugates with dexamethasone-liposomes

Biomacromolecules

Polyion Complex Micelle Based on Albumin–Polymer Conjugates: Multifunctional Oligonucleotide Transfection Vectors for Anticancer Chemotherapeutics

Beilstein Journal of Nanotechnology

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

Accounts of Chemical Research

Nanoparticle–Cell Interactions: Molecular Structure of the Protein Corona and Cellular Outcomes

Physicochemical Properties, Biomolecular Corona, Bioactivity, and Pharmacology-Based Issues of Biopolymers: A Brief Overview

Journal	Data powered by TypesetTrends in Biotechnology
Publisher	Data powered by TypesetElsevier BV
ISSN	0167-7799
Open Access	No