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Physio-chemical optimization of Tannese production by Klebsiella pneumoniae MTCC 7162 using fractional factorial statistical methods
J. Aravind, , K. Jayaraman
Published in
Volume: 10
Issue: 2
Pages: 283 - 290
Tannase an enzyme capable of hydrolyzing tannins was produced by one of our bacterial isolates from tannery wastes and later identified as Klebsiella pneumoniae MTCC 7162. Five nutritional parameters (tannic acid, NaNO3, K2HPO4, KCl and MgSO4.7H2O) and three physical parameters (pH, temperature and agitation) were screened for their effect on increased production of tannase using modeling and design software. Theoretical experimentations of batch mode were considered for fermentor run which resulted in a three fold increase in the tannase production (41.5 U/mL) than the batch studies (14 U/mL) and over all 10 fold increase than non-optimized studies (4 U/mL). © Global Science Publications.
About the journal
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences