Objective: Nature always stands as a golden mark to exemplify the outstanding phenomena of symbiosis. Premna latifolia belonging to genus Premna and family Verbenaceae was one among them. The objective of the present study is to investigate the pharmacognostical and phytochemical evaluation of premna latifolia. Methods: Morphological and microscopical characters of the plant were studied and preliminary photochemical analysis of ethanol, chloroform, petroleum ether and aqueous extracts of these plants were done. Root powder was treated with different reagents and observed for fluorescence under visible light and UV light of short and long wavelength. TLC and HPTLC studies of various extracts of the plant also were done. Results: The powder microscopy showed presence of fibres, parenchyma cells and tracheids. The phytochemical results showed strong presence of triterpenoids and alkaloids with trace amounts of carbohydrates and flavonoids. The physiochemical parameters of root powder were within the limits. The root powder also showed fluorescence. TLC and HPTLC of various extracts of the plant also yielded satisfactory results. Conclusion: The comparative and multidisciplinary approach of the study of Premna latifolia helps in understanding its identification, taxonomical determination and medicinal importance in depth. Further evaluation needs to be carried out on Premna latifolia in order to investigate the obscured areas and their practical clinical applications, which can be used for the welfare of the mankind.