Objective: Quantitative estimation of the phytochemicals viz., alkaloids, terpenoids, phenols and flavonoids were carried out in fifteen different accessions of Ocimum species and was compared with control viz., Ocimum sanctum and O. basilicum to find out the best performing accessions. Methods: The leaf samples were collected from 120 days old plants. The total phenolic content was determined by folin ciocalteau method, total flavonoids content was determined by aluminium chloride method, alkaloid determination using Harborne method and quantification of terpenoids was done according to Morigiwa. Results: The concentration of phenols, flavonoids, alkaloids and terpenoids were found in the range of 31.16-177.44 mg/g gallic acid equivalent, 3.23-34.78 mg/g quercetin equivalent, 11.67-77.33 mg/g and 216.16-350 mg/g respectively. The maximum concentration of flavonoids and phenolics were found in the accession IC201233. The maximum amount of alkaloid content was found in the accession IC381552 and the maximum amount of terpenoids were found in the accessions EC388890, EC338785, IC436153, IC381552 and IC469904. Conclusion: The amount of alkaloids and terpenoids were found to be higher in the accessions when compared to the controls and hence can be utilized to meet various pharmaceutical and industrial purposes.