In this study we have analyzed the chemical composition of 5 locally available underutilized green leafy vegetables (GLV) obtained from Vellore region, namely : Methi, Mint, Lunia, Araaranthus and Tamarind (common names). Aqueous extracts of GLV's were analysed for various chemical components such as minerals, carbohydrates, proteins, phenols, flavonoids. Antioxidant activity of these components have been evaluated by DPPH method and found to be in the order of Mint > Amaranthus > Tamarind > Lunia > Methi. Mineral contents were measured for all the GLV. All the extracts were tested for their antimicrobial efficacy against Gram-positive and Gram-negative microorganisms. Fortification of extracts with probiotic Lactobacillus acidophilus (LAB) was carried out and tested for their antimicrobial property. Upon fortification, it was observed higher antimicrobial property when compared against GLV extracts. This is a novel study carried out for the first time using bio-fortification of phytochemical extracts enhanced LAB strains. © 2015 Scientific Publishers. All Rights Reserved.