Leucas aspera commonly known as ‘Thumbai’ has a wide variety of medical applications. The aqueous extract of Leucas aspera (leaf extract) was assessed for its various in vitro activities such as anti-inflammatory, anti-helminthic, anti-arthritic, anti-oxidant and antibacterial activity. The in vitro Anti-inflammatory activity was studied by human red blood cell (HRBC) membrane stabilization method by using various concentration of L. aspera. The results showed that the HRBC Membrane stabilization activity of the aqueous leaf extract of L. aspera at concentration of 100μg/ml showed 73.25% inhibition of denaturation in hypotonic solution while the standard Diclofenac 100μg/ml showed 79.25% inhibition of denaturation. The crude extracts of the leaves of Leucas aspera showed statistically significant anti-inflammatory activity in in vitro assay.The crude aqueous leaves extract of Leucas aspera also showed that significant anti-helmintic effect causing death of the worm at all the concentrations but the time of death was different in each case. Invitro anti-arthritic activity was carried out by bovine serum protein denaturation method and egg albumin denaturation method. The results suggested that the aqueous extract of L. aspera showed a very good anti-arthritic activity. Antioxidant and Antibacterial activity was also evaluated. The present study concluded that the plant can be formulated in broad spectrum antibiotics and also confirms the traditional uses in pathogenic disease. © 2016, Sphinx Knowledge House. All rights reserved.