Bhaskara Rao K-V

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Ojha V

Preeti, Kumar G

Karthik L.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Biologically Active Products from Nature

Complimentary or natural antioxidant type of alternative medicine is developed worldwide to treat male infertility. The aim of this study is to the extraction of T. arjuna bark and activity against human sperm DNA damage in asthenoteratospermic smoker’s subjects—an in vitro study. All preliminary and antioxidant assays (DPPH, H2O2, and total antioxidant, reducing power activity) were done. T. arjuna bark metal analysis was done with AAS. On the other hand, patients were asked to fill a direct questionnaire about smoking history; 25 infertile smokers were identified as asthenoteratospermic; 34 fertile non-smokers (control) were assessed for semen parameters by CASA, seminal plasma Zinc analysis by AAS, DNA fragmentation by colorimetric method and semen genomic DNA damage inhibition by modified non-enzymatic salting out extraction method. Most of the antioxidants are highly present in the aqueous extract; meanwhile, the major content in this extract is zinc 16 µg/g (Ca = 0.5 µg/g; Se = 2.2 µg/g and Mg = 1.6 µg/g) along with FT-IR peaks which also confirmed the metal presence. The semen parameters in smokers that were noticed are low sperm count and morphological changes. Meanwhile, in the seminal plasma of smokers, zinc and DNA fragmentation results were positively correlated with sperm morphology (p &lt; 0.001). Repaired DNA bands were noticed in the in vitro study of aqueous T. arjuna bark, in smokers’ semen. T. arjuna bark will act as cryo protector as well as great zinc supplementary to maintain sperm motility and morphology in smokers. © 2017, Springer-Verlag GmbH Germany.

Fulltext

3 Biotech

Human sperm DNA damage inhibition and antioxidant activity of T. arjuna bark: an in vitro study

Objective: To analyze the phytochemical composition and in vitro antioxidant properties of aqueous extract of Aerva lanata (A. lanata) stem. Methods: During the preliminary phytochemical analysis, the aqueous extract of A. lanata was screened for the presence of carbohydrates, proteins, phenolic compounds, oil and fats, saponins, flavonoids, alkaloids, tannins and phytosterols. Antioxidant activity of the extract was determined by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity, metal chelating activity, reducing power activity and DNA damage inhibition activity. Analysis of phenolic compounds was performed by Folin-Ciocalteau reagent method and gradient high performance liquid chromatography technique. Results: Preliminary phytochemical analysis exhibited the presence of phenolic compounds, saponins, flavonoids, tannins and phytosterols as major phytochemical groups. The extract exhibited high 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC50= 110.74 μg/mL), metal chelating activity (IC50= 758.17 μg/mL), reducing power activity and DNA damage inhibition efficiency. The extract was reported to possess a high amount of total phenolic content and some of them were identified as gallic acid (3,4,5-OH), apigenin-7-O-glucoside (apigetrin), quercetin-3-O-rutinoside (rutin) and myricetin (3,5,7,3,4,5-OH) by high performance liquid chromatography analysis. The extract was found non toxic towards human erythrocytes in the hemolytic assay (IC50 = 24.89 mg/mL). Conclusions: These results conclud that A. lanata stem possesses high antioxidant activity and can be used for the development of natural and safe antioxidant compounds. © 2013 Hainan Medical College.

Asian Pacific Journal of Tropical Medicine

Phytochemical composition and in vitro antioxidant activity of aqueous extract of Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae)

Cr(VI) (hexavalent) is a very strong oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antioxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant (P

Food and Chemical Toxicology

Aqueous extract of Phyllanthus amarus inhibits chromium(VI)-induced toxicity in MDA-MB-435S cells

Pharmacognosy Communication

Screening of in vitro Cytotoxicity, Evaluation of Hepatoprotective and Oxidative Stress Inhibiting Potential of Clitoria ternata in Carbon Tetrachloride Induced Hepatic Damage in Rats

International Journal of Chemical and Analytical Science

Antimutagenic and antioxidant activity of Ficus benghalensis stem bark and Moringa oleifera root extract

Evaluation of Anti-inflammatory Activity ofFicus benghalensisin Rats

Phytochemical Composition and Antioxidant Activity ofFicus benghalensis(Moraceae) Leaf Extract

Journal	Journal of Biologically Active Products from Nature
Publisher	Informa UK Limited
ISSN	2231-1866
Open Access	0