The research aimed to find the different phytochemical compounds present in the dry and fresh leaves of Ocimum sanctum and its efficiency in removing chromium. The phytochemical property of Ocimum sanctum was studied using the fresh and dry leaves which was ground using morter and pestle that showed the presence of Carboxylic Acid, phenolic substances, carbohydrates, Reducing sugar, Aldehydes, Ketones,Esters, Flavonoids and Proteins. After adding of Ocimum sanctum's leaves to the 50 ppm and stems to 25 ppm of chromium solution shows that the Ocimum sanctum has a great adsorbent property as the concentration of chromium decreases with time. Stems are more efficient in removing chromium than leaves. The research can be used for further studies and development of Ocimum sanctum plant for Bioremediation and pharmacological use.