The present study was to evaluate the antibacterial and antibiofilm activities of various solvent extracts of Alstonia scholaris leaf against biofilm forming bacterial pathogens. The petroleum ether, methanol, acetone and chloroform extracts were examined for phytochemical analysis, agar well diffusion, minimum inhibitory concentration, biofilm inhibitory effects, growth curve and light microscope analysis performed against the biofilm forming bacteria. The total phenolic and flavonoid content were determined by the spectrophotometric method. The quantitative analysis of GC-MS was further examined. Phytochemical screening of various extracts shows the presence of different phytoconstituent. Methanol extracts showed the highest phenolic and flavonoid content than the other extracts. The antibacterial and antibiofilm activities for extracts have been performed against four bacterial pathogens. Almost 90% of inhibited bacterial biofilm formation was concentration-dependent of various extracts at 1mg/ml. Growth curve analysis revealed that all the solvent extracts had no effect on the growth of bacterial pathogens at 1mg/ml concentration when compared to the control. Microscopic analysis of various extracts against the bacterial strains significantly reduced the biofilm formation when compared to the control. The preliminary studies revealed that the methanol extracts of Alstonia scholaris leaf acquire the antibiofilm activity against bacterial pathogen by interrupting the synthesis of bacterial cell wall and inhibition of biofilm formation. © 2018 World Research Association. All rights reserved.